The PSO comes under the administration of the City of Prague
| 1st violin | ||
| Rita Čepurčenko | Concertmaster | |
| Jiří Hurník | Concertmaster | |
| Antonín Pergler | Assistant Concertmaster | |
| Pavel Šafařík | Assistant Concertmaster | |
| Adéla Vondráčková | Assistant Concertmaster | |
| Maria Ericsson Vlach | ||
| Anna Jírovcová | ||
| Kateřina Gemrotová | ||
| Radim Šisler | ||
| Petr Hlaváč | ||
| Pavel Štecher | ||
| Martin Kameš | ||
| Světlana Pechoušková | ||
| Filip Šilar | ||
| František Kosina | ||
| Blanka Fousková | ||
| Jan Mráček | ||
| Josef Riedlbauch | ||
| Matěj Vlk | ||
| Pavel Hrabánek |
| viola | ||
| Pavel Peřina | Principal | |
| Tomáš Duda | ||
| Vladimír Bažant | ||
| Světlana Jahodová | ||
| Alan Melkus | ||
| Oldřich Smola ml. | ||
| Oldřich Smola | ||
| Jan Nykrýn ml. | ||
| Ludmila Topiarzová | ||
| Zuzana Peřinová | ||
| Vladimír Zajačik | ||
| Jiří Zajíc | ||
| Milan Vladyka | ||
| cello | ||
| Miloš Jahoda | Concertmaster | |
| Jan Chuchro | Assistant Concertmaster | |
| Jaroslav Matějka | Assistant Concertmaster | |
| Richard Žemlička | ||
| Roman Stehlík | ||
| Věra Anýžová | ||
| Petr Malíšek | ||
| Petra Holubcová | ||
| Jan Halama | ||
| Václav Fleissig | ||
| Michaela Kučerová | ||
| Jan Klika | ||
| double bass | ||
| Martin Zelenka | Principal | |
| Michal Novák | ||
| Lukáš Verner | ||
| Václav Hoskovec | ||
| Marek Lustig | ||
| David Fendrych | ||
| Vladimír Malík | ||
| Jan Buble | ||
| Jaroslav Himmer |
| flute | ||
| Václav Michálek | Principal | |
| Hana Brožová | ||
| Simona Pingitzer | ||
| Jiří Skuhra | ||
| Martin Klimánek | ||
| Petra Hoďánková |
| piccolo | ||
| Petra Hoďánková |
| oboe | ||
| Liběna Séquardtová | ||
| Jurij Likin | ||
| Radim Kocina | ||
| Jan Hoďánek | ||
| Monika Boušková |
| english horn | ||
| Jan Hoďánek | ||
| Monika Boušková |
| clarinet | ||
| Jaroslav Marek | Principal | |
| Vlastimil Mareš | ||
| Zbyněk Cícha | ||
| Jan Mach |
| bassoon | ||
| Svatopluk Čech | Principal | |
| Luboš Hucek | ||
| Václav Fürbach | ||
| Jaroslav Jedlička | ||
| Petr Němeček |
| trumpet | ||
| Marek Zvolánek | ||
| Jan Fišer | Principal | |
| Josef Řezníček | ||
| Milan Bašta |
| french horn | ||
| Zuzana Rzounková | ||
| František Langweil | ||
| Petr Hernych | Principal | |
| Tomáš Kirschner | ||
| Eva Krajhanzlová | ||
| Jana Adámková | ||
| Petr Chomoucký | ||
| Jiří Fousek |
| trombone | ||
| Jiří Novotný | Principal | |
| Václav Ferebauer | ||
| Julius Nečásek | ||
| Stanislav Penk | ||
| Antonín Keller |
| tuba | ||
| Miroslav Husák |
| harp | ||
| Hana Jouzová | ||
| Magdaléna Šimečková |
| percussion | ||
| Lubor Krása | Principal | |
| Svatopluk Čech ml. | ||
| Martin Kopřiva | ||
| David Řehoř | ||
| Junko Honda |
| keyboard instruments | ||
| Daniela Valtová Kosinová |
| technicians | ||
| Jiří Štěpán | ||
| Josef Císař |
